G-banding: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
'''G-banding''' is one of the chromosome staining techniques that results in banding patterns on chromosomes. It uses the chemical dye, Giemsa, to create distinct chromosome-specific patterns called G-bands. It allows the identification of each chromosome, as well as its different regions, relative to its banding pattern.<ref name="G-banding">Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.</ref> | '''G-banding''' is one of the chromosome staining techniques that results in banding patterns on chromosomes. It uses the chemical dye, Giemsa, to create distinct chromosome-specific patterns called G-bands. It allows the identification of each chromosome, as well as its different regions, relative to its banding pattern.<ref name="G-banding">Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.</ref> | ||
The method for G-banding begins with subjecting the chromosome to a treatment involving trypsin (a proteolytic enzyme), and then staining it with Giemsa. This produces dark-stained bands at the regions with lower G-C content. <ref name="Method ">Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.</ref> | |||
<br> | |||
=== References === | === References === | ||
<references /> | <references /> | ||
Revision as of 11:29, 27 November 2018
G-banding is one of the chromosome staining techniques that results in banding patterns on chromosomes. It uses the chemical dye, Giemsa, to create distinct chromosome-specific patterns called G-bands. It allows the identification of each chromosome, as well as its different regions, relative to its banding pattern.[1]
The method for G-banding begins with subjecting the chromosome to a treatment involving trypsin (a proteolytic enzyme), and then staining it with Giemsa. This produces dark-stained bands at the regions with lower G-C content. [2]