Ribosome intiation: Difference between revisions
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Ribosome Initiation is the first step in the translation of mRNA to a protein. It requires initiation factors IF1, IF2 and IF3 as well as [[GTP|GTP]] for energy and an initiator tRNA known as fMet-tRNA<sup>fMet<ref>Berg J, Tymoczko J L, Gatto G J, Stryer L. Biochemistry. 8th ed. New York. W. H. Freeman and Company. 2015</ref></sup>. Initiation in prokaryotes occurs as follows: | [[Ribosome|Ribosome]] Initiation is the first step in the translation of [[mRNA|mRNA]] to a [[protein|protein]]. It requires [[Initiation_factor|initiation factors]] IF1, IF2 and IF3 as well as [[GTP|GTP]] for energy and an initiator [[tRNA|tRNA]] known as fMet-tRNA<sup>fMet<ref>Berg J, Tymoczko J L, Gatto G J, Stryer L. Biochemistry. 8th ed. New York. W. H. Freeman and Company. 2015</ref></sup>. Initiation in [[prokaryotes|prokaryotes]] occurs as follows: | ||
#IF1 and IF3 bind to the free 30S subunit of the [[Composition of prokaryotic ribosome|70S ribosome]]. | #IF1 and IF3 bind to the free 30S subunit of the [[Composition of prokaryotic ribosome|70S ribosome]]. | ||
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#The 30S subunit then binds to the 16S complementary region of the mRNA strand. | #The 30S subunit then binds to the 16S complementary region of the mRNA strand. | ||
#A charged initiator tRNA can now bind which causes the release of IF3. | #A charged initiator tRNA can now bind which causes the release of IF3. | ||
#The 50S subunit binds forming the ribosome, displacing IF1 and IF 2 and hydrolysing GTP to GDP. This step is the energy consuming step<ref>Berg J, Tymoczko J L, Gatto G J, Stryer L. Biochemistry. 8th ed. New York. W. H. Freeman and Company. 2015</ref>. | #The 50S subunit binds forming the ribosome, displacing IF1 and IF 2 and hydrolysing [[GTP|GTP]] to [[GDP|GDP]]. This step is the energy consuming step<ref>Berg J, Tymoczko J L, Gatto G J, Stryer L. Biochemistry. 8th ed. New York. W. H. Freeman and Company. 2015</ref>. | ||
<br> | === References<br> === | ||
<references /> | <references /> | ||
Latest revision as of 13:32, 30 November 2018
Ribosome Initiation is the first step in the translation of mRNA to a protein. It requires initiation factors IF1, IF2 and IF3 as well as GTP for energy and an initiator tRNA known as fMet-tRNAfMet[1]. Initiation in prokaryotes occurs as follows:
- IF1 and IF3 bind to the free 30S subunit of the 70S ribosome.
- A GTP/IF2 complex then binds to the 30S subunit.
- The 30S subunit then binds to the 16S complementary region of the mRNA strand.
- A charged initiator tRNA can now bind which causes the release of IF3.
- The 50S subunit binds forming the ribosome, displacing IF1 and IF 2 and hydrolysing GTP to GDP. This step is the energy consuming step[2].