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K<sub>m</sub> = Michaelis constant
Michaelis constant is the substrate concentration at which the reaction velocity is equal to half the maximal velocity of the reaction.<sup></sup>


K<sub>m</sub> = the value of [S] that causes V= ½ V<sub>max</sub> *  
K<sub>m</sub> = Michaelis constant<sup></sup>
 
K<sub>m</sub> = the value of substrate concentration[S] that causes V= ½ V<sub>max</sub> *  


The units of K<sub>m</sub> are M, [[Concentration|concentration]].  
The units of K<sub>m</sub> are M, [[Concentration|concentration]].  


K<sub>m</sub> indicates the [[javascript:void(0);/*1512127072657*/|affinity]] of the enzyme for its substrate and thus the stability of the [[Enzyme-Substrate Complex|Enzyme-Substrate Complex]].  
K<sub>m</sub> indicates the [[Javascript:void(0);/*1512127072657*/|affinity]] of the enzyme for its substrate and thus the stability of the [[Enzyme-Substrate Complex|Enzyme-Substrate Complex]].<br>
 
Velocity is related to K<sub>m&nbsp;</sub>through the Michaelis &amp; Menten equation:&nbsp;v = (V<sub>max</sub> [S])/(K<sub>m</sub> + [S]).<br>
 
*[[Vmax|V<sub>max</sub>]] = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration.
 
[S] = [[Substrate|substrate]] concentration.<br>


High K<sub>m</sub> demonstrates a low affinity; Low Km demonstrates a high affinity.  
A reaction with a low Michaelis constant value indicates a low binding affinity, where as a large value indicates a high binding affinity.&nbsp;


Velocity is related to K<sub>m&nbsp;</sub>through the Michaelis &amp; Menten equation:&nbsp;v = (V<sub>max</sub> [S])/(K<sub>m</sub> + [S]).<br>
<br>  


*[[Vmax|V<sub>max</sub>]] = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration.
= References  =


[S] = [[Substrate|substrate]] concentration.<br>
Michaelis, L&nbsp;; Menten, M L&nbsp;; Johnson, K A&nbsp;; Goody, R S. The original Michaelis constant: translation of the 1913 Michaelis-Menten paper,<br>''Biochemistry,'' 04 October 2011, Vol.50(39), pp.8264-9

Revision as of 16:35, 6 December 2018

Michaelis constant is the substrate concentration at which the reaction velocity is equal to half the maximal velocity of the reaction.

Km = Michaelis constant

Km = the value of substrate concentration[S] that causes V= ½ Vmax *

The units of Km are M, concentration.

Km indicates the affinity of the enzyme for its substrate and thus the stability of the Enzyme-Substrate Complex.

Velocity is related to Km through the Michaelis & Menten equation: v = (Vmax [S])/(Km + [S]).

  • Vmax = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration.

[S] = substrate concentration.

A reaction with a low Michaelis constant value indicates a low binding affinity, where as a large value indicates a high binding affinity. 


References

Michaelis, L ; Menten, M L ; Johnson, K A ; Goody, R S. The original Michaelis constant: translation of the 1913 Michaelis-Menten paper,
Biochemistry, 04 October 2011, Vol.50(39), pp.8264-9

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