RNA polymerase II: Difference between revisions

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RNA poymerase II is an [[enzyme|enzyme]] found in eukaryotic cells.It is responsible for transcribing all protein-coding genes as well as several small [[Nucleus|nuclear]] [[RNA|RNAs]], such as U1,U2 and U3.
Found in Eukaryotic cells, RNA polymerase II is used&nbsp;to transcribe all protein-encoding genes, and in addition, many of the small nucleolar RNAs (snoRNA)&nbsp;and the majority of the micro RNAs (miRNA)<ref>Wilhelm E et al (2012)'Probing Endogenous RNA Polymerase II Pre-initiation Complexes by Electrophoretic Mobility Shift Assay', Methods Mol Biol. 809:63-74</ref>. RNA polymerase II cannot transcribe DNA on its own: the many&nbsp;elements that make up the pre-initiation complex (PIC) must assemble at the site of the promoter in order for RNA polymerase II to be recruited and begin transcription<ref>Seizl M et al. (2011). A Conserved GA Element in TATA-Less RNA Polymerase II Promoters. PLoS One. 6 (11), e27595</ref>. The PIC is made up of Transcription Factors: TFIID, TFIIA, TFIIB, TFIIF, TFIIE, TFIIH<ref>D. B. Nikolov et al. (1997). RNA polymerase II transcription initiation: A structural view. PNAS. 94 (1), 15-22.</ref>.
 
 
 
References
 
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Revision as of 17:37, 26 November 2011

Found in Eukaryotic cells, RNA polymerase II is used to transcribe all protein-encoding genes, and in addition, many of the small nucleolar RNAs (snoRNA) and the majority of the micro RNAs (miRNA)[1]. RNA polymerase II cannot transcribe DNA on its own: the many elements that make up the pre-initiation complex (PIC) must assemble at the site of the promoter in order for RNA polymerase II to be recruited and begin transcription[2]. The PIC is made up of Transcription Factors: TFIID, TFIIA, TFIIB, TFIIF, TFIIE, TFIIH[3].


References

  1. ↑ Wilhelm E et al (2012)'Probing Endogenous RNA Polymerase II Pre-initiation Complexes by Electrophoretic Mobility Shift Assay', Methods Mol Biol. 809:63-74
  2. ↑ Seizl M et al. (2011). A Conserved GA Element in TATA-Less RNA Polymerase II Promoters. PLoS One. 6 (11), e27595
  3. ↑ D. B. Nikolov et al. (1997). RNA polymerase II transcription initiation: A structural view. PNAS. 94 (1), 15-22.
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