Non-denaturing gel: Difference between revisions
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Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis ([[SDS polyacrylamide-gel electrophoresis|PAGE]]). Detergents are used at a minimum to lyse [[ | Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis ([[SDS polyacrylamide-gel electrophoresis|PAGE]]). Detergents are used at a minimum to lyse [[Cell|cell]] membranes and no charged denaturing agents are used. The molecules being separated remain folded<ref>http://www.ap-lab.com/native_gels.htm</ref>, however separation does not occur as predictibly as those run in a denaturing gel as shape does not usually relate to size and charge may not be as evenly distributed compared to proteins run in [[SDS polyacrylamide-gel electrophoresis]]. | ||
Revision as of 09:20, 2 December 2011
Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis (PAGE). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded[1], however separation does not occur as predictibly as those run in a denaturing gel as shape does not usually relate to size and charge may not be as evenly distributed compared to proteins run in SDS polyacrylamide-gel electrophoresis.