Luciferase: Difference between revisions
Jump to navigation
Jump to search
Created page with "This a group of oxidative enzymes that convert luciferin to oxyluciferin in the presence of light leading to the generation of light. The name is derived from the word 'lucifer' ..." |
No edit summary |
||
| Line 1: | Line 1: | ||
This a group of oxidative enzymes that convert luciferin to oxyluciferin in the presence of light leading to the generation of light. The name is derived from the word 'lucifer' which means light bearer and is useful in the pyrosequencing technique used in new generation DNA sequencing | This a group of oxidative enzymes that convert luciferin to oxyluciferin in the presence of light leading to the generation of light. The name is derived from the word 'lucifer' which means light bearer and is useful in the pyrosequencing technique used in new generation DNA sequencing <ref name="1">Gould SJ, Subramani S (November 1988). "Firefly luciferase as a tool in molecular and cell biology". Anal. Biochem. 175 (1): 5–13. doi:10.1016/0003-2697(88)90353-3. PMID 3072883.</ref> | ||
luciferin + ATP → luciferyl adenylate + PPi<br>luciferyl adenylate + O2 → oxyluciferin + AMP + light<br> | luciferin + ATP → luciferyl adenylate + PPi<br>luciferyl adenylate + O2 → oxyluciferin + AMP + light<br>References. | ||
Gould SJ, Subramani S (November 1988). "Firefly luciferase as a tool in molecular and cell biology". Anal. Biochem. 175 (1): 5–13. doi:10.1016/0003-2697(88)90353-3. PMID 3072883. | |||
Revision as of 11:50, 19 October 2012
This a group of oxidative enzymes that convert luciferin to oxyluciferin in the presence of light leading to the generation of light. The name is derived from the word 'lucifer' which means light bearer and is useful in the pyrosequencing technique used in new generation DNA sequencing Cite error: Invalid <ref> tag; name cannot be a simple integer. Use a descriptive title
luciferin + ATP → luciferyl adenylate + PPi
luciferyl adenylate + O2 → oxyluciferin + AMP + light
References.
Gould SJ, Subramani S (November 1988). "Firefly luciferase as a tool in molecular and cell biology". Anal. Biochem. 175 (1): 5–13. doi:10.1016/0003-2697(88)90353-3. PMID 3072883.