Hydrophobic interaction chromatography: Difference between revisions
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Used to seperate proteins that have different properties, i.e [[ | Used to seperate proteins that have different properties, i.e [[Proteins|proteins]] that are [[Hydrophobic|hydrophobic]] are seperated from those that are [[Hydrophillic|hydrophillic]]. | ||
[[Proteins|Proteins]] with hydrophobic properties bind to the hydrophobic column whilst hydrophilic proteins are eluted out. | [[Proteins|Proteins]] with hydrophobic properties bind to the hydrophobic column whilst hydrophilic proteins are eluted out. The columns are packed with lots of beads from which hydrophobic side chains protrude. These hydrophobic side chains then selectively disable proteins containing exposed hydrophobic regions. <references /> Alberts et al (2008) Molecular Biology of the cell, fifth edition. | ||
To elute the hydrophobic protein, you can make the solvent more hydrophobic. | To elute the hydrophobic protein, you can make the solvent more hydrophobic. | ||
Revision as of 12:23, 30 November 2012
Used to seperate proteins that have different properties, i.e proteins that are hydrophobic are seperated from those that are hydrophillic.
Proteins with hydrophobic properties bind to the hydrophobic column whilst hydrophilic proteins are eluted out. The columns are packed with lots of beads from which hydrophobic side chains protrude. These hydrophobic side chains then selectively disable proteins containing exposed hydrophobic regions. Alberts et al (2008) Molecular Biology of the cell, fifth edition.
To elute the hydrophobic protein, you can make the solvent more hydrophobic.