Michaelis menten equation: Difference between revisions
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'''V'''<sub>'''max'''</sub> is the maximum rate of an enzyme reaction, occurs when all substrate is saturated.<br> | '''V'''<sub>'''max'''</sub> is the maximum rate of an enzyme reaction, occurs when all substrate is saturated.<br> | ||
'''K<sub>m</sub>''' is the Michaelis-Menten constant and is the substrate concentration at half V<sub>max. </sub> | '''K<sub>m</sub>''' is the Michaelis-Menten constant and is the substrate concentration at half V<sub>max. </sub><br> | ||
< | <sub></sub>V<sub>max</sub> and K<sub>m</sub> can also be shown on a graph, the graph which shows this best is a '''double reciprocal plot'''. You can obtain the results by plotting 1/V against 1/[S] <ref>Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163</ref>. | ||
=== References === | |||
=== References === | |||
<references /> | <references /> | ||
Revision as of 18:25, 14 November 2010
The Michaelis-Menten equation is used to work out the rate of enzyme reactions and is written as follows:
V = Vmax [S]
Km + [S]
Vmax is the maximum rate of an enzyme reaction, occurs when all substrate is saturated.
Km is the Michaelis-Menten constant and is the substrate concentration at half Vmax.
Vmax and Km can also be shown on a graph, the graph which shows this best is a double reciprocal plot. You can obtain the results by plotting 1/V against 1/[S] [1].
References
- ↑ Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163