EcoR1: Difference between revisions
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''Eco''R1 is a restriction endonuclease (or [[Restriction enzyme|restriction enzyme]]) that cleaves double stranded DNA at the sequence GTTAAC, making staggered cuts, which leave single stranded complementary overhangs, called ‘[[‘sticky’ ends|sticky ends]]’<ref>Molecular Cell Biology. 4th edition. Lodish H, Berk A, Zipursky SL, et al. New York: W. H. Freeman; 2000</ref>. The [[Palindromic sequence|palindromic sequence]], GTTAAC, occurs in DNA approximately every 46 bases, 4.096 Kb<ref name="null">Modern Genetic Analysis. Griffiths AJF, Gelbart WM, Miller JH, et al. New York: W. H. Freeman; 1999.</ref>. The ''Eco''R1 enzyme is found in some strains of ''[[E. coli|E. Coli]]'', it is used by the cell for defence, to cleave foreign DNA. Other restriction enzymes can be found in other bacteria species. | ''Eco''R1 is a restriction endonuclease (or [[Restriction enzyme|restriction enzyme]]) that cleaves double stranded DNA at the sequence GTTAAC, making staggered cuts, which leave single stranded complementary overhangs, called ‘[[‘sticky’ ends|sticky ends]]’<ref>Molecular Cell Biology. 4th edition. Lodish H, Berk A, Zipursky SL, et al. New York: W. H. Freeman; 2000</ref>. The [[Palindromic sequence|palindromic sequence]], GTTAAC, occurs in DNA approximately every 46 bases, 4.096 Kb<ref name="null">Modern Genetic Analysis. Griffiths AJF, Gelbart WM, Miller JH, et al. New York: W. H. Freeman; 1999.</ref>. The ''Eco''R1 enzyme is found in some strains of ''[[E. coli|E. Coli]]'', it is used by the cell for defence, to cleave foreign DNA. Other restriction enzymes can be found in other bacteria species.<br> | ||
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Latest revision as of 04:16, 25 October 2013
EcoR1 is a restriction endonuclease (or restriction enzyme) that cleaves double stranded DNA at the sequence GTTAAC, making staggered cuts, which leave single stranded complementary overhangs, called ‘sticky ends’[1]. The palindromic sequence, GTTAAC, occurs in DNA approximately every 46 bases, 4.096 Kb[2]. The EcoR1 enzyme is found in some strains of E. Coli, it is used by the cell for defence, to cleave foreign DNA. Other restriction enzymes can be found in other bacteria species.