Taq polymerase: Difference between revisions
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Taq polymerase is an enzyme found in ''[[Thermus aquaticus|Thermus aquaticus]]'', an organism which live in environments of extremely high temperatures, such as [[Hot springs|hot springs]]. It is an enzyme commonly used in the [[PCR|Polymerase Chain Reaction]] (PCR) as it can withstand the required high temperatures of the [[Thermo Cycler|Thermo Cycler]]. | Taq polymerase is an enzyme found in ''[[Thermus aquaticus|Thermus aquaticus]]'', an organism which live in environments of extremely high temperatures, such as [[Hot springs|hot springs]]. It is an enzyme commonly used in the [[PCR|Polymerase Chain Reaction]] (PCR) as it can withstand the required high temperatures of the [[Thermo Cycler|Thermo Cycler]]. | ||
During PCR, Taq polymerase is used to extend the [[Primers|primers]]. Alternatively, [[Pfu|Pfu]] is another thermostable DNA polymerase that may be used in PCR, this has a proof reading ability and is therfore more accurate. <ref name="Methods Mol Biol. 2011;682:65-75.">Whether using Taq polymerase or Pfu, either overhangs or blunt ends will be created respectively.</ref>. <references /> | |||
Revision as of 16:35, 15 November 2010
Taq polymerase is an enzyme found in Thermus aquaticus, an organism which live in environments of extremely high temperatures, such as hot springs. It is an enzyme commonly used in the Polymerase Chain Reaction (PCR) as it can withstand the required high temperatures of the Thermo Cycler.
During PCR, Taq polymerase is used to extend the primers. Alternatively, Pfu is another thermostable DNA polymerase that may be used in PCR, this has a proof reading ability and is therfore more accurate. [1].
- ↑ Whether using Taq polymerase or Pfu, either overhangs or blunt ends will be created respectively.