SDS polyacrylamide-gel electrophoresis

 SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins due to their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied. Alberts,Bruce et al(2008) Molecular Biology of the Cell(5th edition) Garland Science Pg 517