SDS polyacrylamide-gel electrophoresis

SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins due to their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied . Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larher in size.