Rho independent terminators

Rho- independent termination ( also known as intrinsic termination) is a process carried out in prokaryotic organisms as a mechanism for stopping Transcription. the mRNA molecule has a sequence of base pairs with a high proportion of Cytosine and Guanine bases, these are able to strongly bond to one another with 3 hydrogen bonds forming a RNA duplex in a stable hairpin structure. succeeding the hairpin structure is a chain of Uracil bases, which only form weak bonds with Adenine. the transcription of the uracil sequence is coupled with a protein, located on the RNA polymerase, binding to the hairpin structure causing the RNA polymerase to temporarily stop. The temporary lapse and weak uracil- adenine bonds cause the hairpin structure to unwind and dissociate from the RNA polymerase, thus terminating transcription.