Centrifugation

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Centrifugation can be used to break up cells into fragments and is the first step in most fractionations. The suspension of cells produced from this process is called a homogenate or extract.

A preparative ultracentrifuge separates cell components by size and density by generating enormous centrifugal forces. It does this by rotating extracts of broken cells at high speeds. [1]

References

  1. ↑ Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. (2008: 510-511) Molecular Biology of the Cell, 5th edition, Abingdon: Garland Science
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