Michaelis constant
Km = Michaelis constant
Km = the value of [S] that causes V= ½ Vmax *
The units of Km are M, concentration
Km indicates the affinity of the enzyme for it's substrate and thus the stability of the Enzyme-Substrate Complex.
High Km demonstrates a low affinity; Low Km demonstrates a high affinity.
velocity is related to Km through the Michaelis & Menten equation: v = (Vmax [S])/(Km + [S])
References:
<u[1]
- Vmax = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration
[S] = substrate concentration
- ↑ Lakey, J. 2012. BGM1002 Lectures 6&7&8: Enzymes. [lecture]. October 15. Newcastle: DSLT. Date of presentation/lecture. Location: Venue. (if available)