SDS polyacrylamide-gel electrophoresis

 SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins due to their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied.(Alberts,Bruce et al, 2008 Pg 517)