Pfu

Pfu

Pfu polymerase is an enzyme isolated from the organism Pyrococcus furiosus, a hyperthermophilic archaeon. This organism can survive temperatures of up to one hundred degrees celcius, therefore is very apt for use in the <a href="PCR">PCR</a> reaction, as, unlike <a href="DNA polymerase">DNA polymerase</a>, it can survive and function at the high temperatures the process operates at. It may be preferred to the use of <a href="Taq polymerase">Taq polymerase </a>(another thermostable enzyme) as pfu has 3'-5' proof reading activity, improving the accuracy of copying <a href="DNA">DNA</a>.