Pyrococcus furiosis

 

Pyrococcus Furiosus

Pyrococcus furiosus is an hyperthermophilic organism found in submarine vents, its DNA polymerase is thus stable at very high temperatures (thermostable). Pfu DNA polymerase is an archeal DNA polymerase so it is classed as a Family B polymerase. Pfu polymerase is especially useful as it has a 3' to 5' proofreading exonuclease ability for excision of an incorrect DNA base pair after which the polymerase re-inserts the correct base pair Edward M. Kennedy, Christopher Hergott, Stephen Dewhurst, and Baek Kim. (2009) The Mechanistic Architecture of the Thermostable Pyrococcus Furiosus Family B DNA Polymerase Motif A and its Interaction with dNTP Substrate. Journal of Biochemistry 48(47): 1116-11168.

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