Non-denaturing gel
Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis (PAGE). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded[1], however separation does not occur as predictibly as those run in a denaturing gel.
Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis (PAGE). SDS is not used and detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used. The molecules being separated remain folded[1], however separation does not occur as predictibly as those run in a denaturing gel, as shape does not usually relate to size and charge may not be as evenly distributed compared to proteins run in SDS_polyacrylamide-gel_electrophoresis.