Tween 20

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Tween 20 is a transparent viscous liquid that is commonly used in Molecular Biology as a potent nonionic detergent, emulsifier and as a blocking agent in immunoassays, such as Western Blotting [1]. Other uses of Tween 20 include removal of membrane peripheral proteins and lysis of mammalian cells[2].


  1. Sigma-Aldrich (n.d). Tween 20.fckLRhttp://www.sigmaaldrich.com/catalog/product/sigma/p9416?lang=en&region=GB. [Last accessed 20/11/13]
  2. Sigma-Aldrich (n.d). Tween 20.fckLRhttp://www.sigmaaldrich.com/catalog/product/sigma/p9416?lang=en&region=GB. [Last accessed 20/11/13]