DNA Template Strand (also known as the anti-sense strand), is one of the strands of DNA that is present after being unwound by the enzyme DNA helicase during transcription of a gene. Ribonucleotide Triphosphates (rNTPs) then align along the sense anti-sense strand by Watson-Crick base pairing. In eukaryotes the ribonucleotides are then joined together by RNA polymerase II (in prokaryotes, this is just RNA polymerase) to form a pre-mRNA molecule that is complementary to the anti-sense strand. Transcription ends when the RNA polymerases reaches a 'stop' codon. In eukaryotes only, splicing will then take place in order to remove any non-coding regions of DNA (introns) to give the final mRNA strand. The mRNA then leaves the nucleus through a nuclear pore and is translated by ribosomes to form a sequence of amino acids and thus a protein.
- ↑ Lodish H., Kaiser CA., Bretscher A., Amon A., Berk A., Krieger M., Ploegh H and Scott MP (2012) Molecular Cell Biology, 7th edition, New York: WH Freeman page:124