Pyrosequencing

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Pyrosequencing (also known as 454-sequencing) is a method of DNA Sequencing. It involves using an enzyme called luciferase, amongst others, to determine the nucleotide sequence based upon the light emitted when a correct dNTP binds to a template.


[1]

  1. Ronaghi M. Pyrosequencing Sheds Light On DNA Sequencing, Genome Res. 2001 11: 3-11