'sticky' ends

From The School of Biomedical Sciences Wiki
(Difference between revisions)
Jump to: navigation, search
(Created page with " Also known as cohesive ends, sticky ends are formed when a double stranded DNA fragment is asymmetrically cleaved by certain restriction enzymes such as EcoRI. These e...")
 
(Added some links.)
 
Line 1: Line 1:
&nbsp;Also known as cohesive ends, sticky ends are formed when a double stranded DNA fragment is asymmetrically cleaved by certain restriction enzymes such as&nbsp;EcoRI. These ends will be complementary to each other so once paired two DNA fragments can be joined together permanently by DNA ligase which catalyses the formation of bonds between the sugar-phosphate groups between the fragments<sup><ref>Benjamin A. Pierce. Genetics: A Conceptual Approach. Fifth edition. New York: W. H. Freeman. 2017.</ref></sup><sup></sup>.  
+
Also known as cohesive ends, sticky ends are formed when a double stranded [[DNA|DNA]] fragment is asymmetrically cleaved by certain [[restriction enzymes|restriction enzymes]] such as&nbsp;[[EcoRI|EcoRI]]. These ends will be complementary to each other so once paired two DNA fragments can be joined together permanently by [[DNA ligase|DNA ligase]] which catalyses the formation of bonds between the [[sugar-phosphate groups|sugar-phosphate groups]] between the fragments<sup><ref>Benjamin A. Pierce. Genetics: A Conceptual Approach. Fifth edition. New York: W. H. Freeman. 2017.</ref></sup><sup></sup>.  
  
This technique is often used in recombinant DNA technology.  
+
This technique is often used in [[recombinant DNA technology|recombinant DNA technology]].<br>
  
<br>
+
=== References ===
 
+
=== References===
+
  
 
<references />
 
<references />

Latest revision as of 18:38, 4 December 2018

Also known as cohesive ends, sticky ends are formed when a double stranded DNA fragment is asymmetrically cleaved by certain restriction enzymes such as EcoRI. These ends will be complementary to each other so once paired two DNA fragments can be joined together permanently by DNA ligase which catalyses the formation of bonds between the sugar-phosphate groups between the fragments[1].

This technique is often used in recombinant DNA technology.

References

  1. Benjamin A. Pierce. Genetics: A Conceptual Approach. Fifth edition. New York: W. H. Freeman. 2017.
Personal tools
Namespaces
Variants
Actions
Navigation
Toolbox