‘sticky’ ends

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A [[Restriction enzyme|restriction enzyme]] can cut [[DNA|DNA]] at a specific sequence of [[Nucleotides|nucleotides]] usually 4, 6 or 8 [[Nucleotides|nucleotides]] long. This may result in [[Blunt ends|symmetrical cleavage]] leading to [[Blunt ends|blunt ends]] or [['sticky' ends|assymetrical cleavage]] causing [[‘sticky’ ends|sticky ends]]. A [[‘sticky’ ends|"sticky" end is]] produced when the [[Restriction enzyme|restriction enzyme]] cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of [[DNA|DNA]] that will have some [[Nucleotides|nucleotides]] without any complementary bases. A [[Restriction enzyme|restriction enzyme]] will only cut at a specific sequence, and it recognises palindromic sequences that is, sequences that are the same whether they are read forwards or backwards. These "sticky" ends allow the insertion of 'foreign' DNA into the host [[Genome|genome]]. By using the same restriction [[Enzyme|enzyme]] to cut a [[Plasmid|plasmid]], for example, complementary bases of the plasmid can pair with those of the host DNA. The [[Genes|genes]] carried on the plasmid will now be incorporated into the hosts genome.  
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A [[Restriction enzyme|restriction enzyme]] can cut [[DNA|DNA]] at a specific sequence of [[Nucleotides|nucleotides]] usually 4, 6 or 8 [[Nucleotides|nucleotides]] long. This may result in [[Blunt ends|symmetrical cleavage]] leading to [[Blunt ends|blunt ends]] or [['sticky' ends|assymetrical cleavage]] causing '[['sticky' ends|sticky' ends]]. A 'sticky' end is produced when the [[Restriction enzyme|restriction enzyme]] cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the [[Complementary strand|complementary strand]]. This will produce two ends of [[DNA|DNA]] that will have some [[Nucleotides|nucleotides]] without any complementary bases. A [[Restriction enzyme|restriction enzyme]] will only cut at a specific sequence and it recognises [[Palindromic sequence|palindromic sequence]] that is, sequences that are the same whether they are read forwards or backwards (For example words like Hannah and Race car are palindromes). These 'sticky' ends allow the insertion of 'foreign' DNA into the host [[Genome|genome]]. By cutting the plasmid with the same restriction [[Enzyme|enzyme]], the same 'sticky ends' are produced. For example, complementary bases of the [[Plasmid|plasmid can]] pair with those of the host DNA and form hydrogen bonds which anneal the two strands together. However, there will still be nicks in the [[Phosphodiester bond|phosphodiester bonds which]] form the rigid phosphate backbone of DNA. In this scenario [[DNA ligase|DNA ligase]] can be added which will form the phosphodiester bonds between the [[Recombinant DNA Technology|recombinant strands]]. The [[Genes|genes]] carried on the plasmid will now be incorporated into the host's genome, creating a recombinant plasmid. However, the gene of interest can sometimes be inserted into the plasmid in the wrong orientation.  
  
Also see [[Blunt end|"blunt" ends]].
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These steps are commonly used in the lab<ref>http://www.scienceaid.co.uk/biology/genetics/engineering.html</ref>.
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For example:
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…..GAATTC…..<br>…..CTTAAG…..<br>After using restiction enzymes to cut at specific sites:
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…..G &nbsp; &nbsp;AATTC…..<br>…..CTTAA &nbsp; &nbsp;G…..<br>
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Also see [[Blunt end|"blunt" ends]].  
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=== References  ===
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<references />

Latest revision as of 07:07, 23 October 2018

A restriction enzyme can cut DNA at a specific sequence of nucleotides usually 4, 6 or 8 nucleotides long. This may result in symmetrical cleavage leading to blunt ends or assymetrical cleavage causing 'sticky' ends. A 'sticky' end is produced when the restriction enzyme cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of DNA that will have some nucleotides without any complementary bases. A restriction enzyme will only cut at a specific sequence and it recognises palindromic sequence that is, sequences that are the same whether they are read forwards or backwards (For example words like Hannah and Race car are palindromes). These 'sticky' ends allow the insertion of 'foreign' DNA into the host genome. By cutting the plasmid with the same restriction enzyme, the same 'sticky ends' are produced. For example, complementary bases of the plasmid can pair with those of the host DNA and form hydrogen bonds which anneal the two strands together. However, there will still be nicks in the phosphodiester bonds which form the rigid phosphate backbone of DNA. In this scenario DNA ligase can be added which will form the phosphodiester bonds between the recombinant strands. The genes carried on the plasmid will now be incorporated into the host's genome, creating a recombinant plasmid. However, the gene of interest can sometimes be inserted into the plasmid in the wrong orientation.

These steps are commonly used in the lab[1].

For example:

…..GAATTC…..
…..CTTAAG…..
After using restiction enzymes to cut at specific sites:

…..G    AATTC…..
…..CTTAA    G…..

Also see "blunt" ends.

References

  1. http://www.scienceaid.co.uk/biology/genetics/engineering.html
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