‘sticky’ ends

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A [[Restriction enzyme|restriction enzyme]] can cut [[DNA|DNA]] at a specific sequence of [[Nucleotides|nucleotides]] usually 4, 6 or 8 nucleotides long. This may result in symmetrical cleavage and [["blunt" ends]] or assymetrical cleavage and "sticky" ends. A "sticky" end is produced when the [[Restriction_enzyme|restriction enzyme]] cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of DNA that will have some nucleotides without any complementary bases. A restriction enzyme will only cut at a specific sequence, and since DNA is [[Palindromic sequence|palindromic]] the end of one sequence is the same as the opposite end of the [[Complementary sequence|complementary sequence]]. 
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A [[Restriction enzyme|restriction enzyme]] can cut [[DNA|DNA]] at a specific sequence of [[Nucleotides|nucleotides]] usually 4, 6 or 8 [[Nucleotides|nucleotides ]]long. This may result in [[Blunt ends|symmetrical cleavage]] leading to [[Blunt ends|"blunt" ends]] or [[‘sticky’_ends|assymetrical cleavage ]]causing "sticky" ends. A "sticky" end is produced when the [[Restriction enzyme|restriction enzyme]] cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of [[DNA|DNA ]]that will have some [[Nucleotides|nucleotides ]]without any complementary bases. A [[Restriction_enzyme|restriction enzyme ]]will only cut at a specific sequence, and since [[DNA|DNA ]]is [[Palindromic sequence|palindromic]] the end of one sequence is the same as the opposite end of the [[Complementary sequence|complementary sequence]].  Also see [[blunt end|"blunt" ends]]

Revision as of 14:44, 16 October 2012

A restriction enzyme can cut DNA at a specific sequence of nucleotides usually 4, 6 or 8 nucleotides long. This may result in symmetrical cleavage leading to "blunt" ends or assymetrical cleavage causing "sticky" ends. A "sticky" end is produced when the restriction enzyme cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of DNA that will have some nucleotides without any complementary bases. A restriction enzyme will only cut at a specific sequence, and since DNA is palindromic the end of one sequence is the same as the opposite end of the complementary sequence.  Also see "blunt" ends

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