Bacterial Gene Transfer

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There are 3 Types of Gene Transfer in [[Bacteria|Bacteria]] <ref>Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Bartlett learning (Chapter 9 Genetics of Bacteria and their viruses)</ref>&nbsp;namely:  
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There are 3 Types of Gene Transfer in [[Bacteria|Bacteria]] <ref>Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Bartlett learning (Chapter 9 Genetics of Bacteria and their viruses)</ref>&nbsp;namely:  
  
 
#Transformation  
 
#Transformation  
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This&nbsp;is the transfer of genes from&nbsp;'''Free [[DNA|DNA]] Molecules '''in the surrounding medium into a recipiant cell.  
 
This&nbsp;is the transfer of genes from&nbsp;'''Free [[DNA|DNA]] Molecules '''in the surrounding medium into a recipiant cell.  
  
The main experiment linked to this was Griffiths work with smooth virilant (Deadly) strain of Streptococcus pneumonia and rough (non-deadly) strain on Mice.<br>When the smooth strain was treated with heat it was killed and so when injected into the mouse it was not deadly.<br>However when the 'Heat-Killed' smooth strain and the rough (non-deadly) strain was mixed and injected together the mouse contracted pneumonia. This shows that the smooth strain has ''''TRANSFORMED' '''the rough strain so it becomes deadly when they are mixed. In other words, the rough strain cells had taken up free DNA of the 'killed' smooth strain and thus had produced a protective coating around themselves thus, appearing 'smooth'.
+
The main experiment linked to this was Griffiths work with smooth virilant (Deadly) strain of Streptococcus pneumonia and rough (non-deadly) strain on Mice.<br>When the smooth strain was treated with heat it was killed and so when injected into the mouse it was not deadly.<br>However when the 'Heat-Killed' smooth strain and the rough (non-deadly) strain was mixed and injected together the mouse contracted pneumonia. This shows that the smooth strain has ''''TRANSFORMED' '''the rough strain so it becomes deadly when they are mixed. In other words, the rough strain cells had taken up free DNA of the 'killed' smooth strain and thus had produced a protective coating around themselves thus, appearing 'smooth'.  
  
For transformation to occur, the recipient cell has to possess 'competence' which is the ability to take up free DNA fragments by an organism. Competence occurs naturally in many bacteria but it can be induced in bacteria when it does not naturally occur. This can be done by treatment with Calcium Chloride CaCl<sub>2</sub> as is the case with Escherichia coli.
+
For transformation to occur, the recipient cell has to possess 'competence' which is the ability to take up free DNA fragments by an organism. Competence occurs naturally in many bacteria but it can be induced in bacteria when it does not naturally occur. This can be done by treatment with Calcium Chloride CaCl<sub>2</sub> as is the case with Escherichia coli.  
  
 
<br>
 
<br>
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=== <u>Conjugation</u>  ===
 
=== <u>Conjugation</u>  ===
  
<u></u>Transference of genes via '''direct cell to cell '''interactions.<br>This methods exchanged [[Plasmid|Plasmids]] (Very uncommon to transfer chromosomal DNA)  
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<u></u>Transference of genes via '''direct cell to cell '''interactions.<br>This method involves&nbsp;exchange of &nbsp;[[Plasmid|Plasmids]] (Very uncommon to transfer chromosomal DNA)  
  
Bacteria of donor cell has a [[Pilus|Pilus]] (protein hair like apendage) on its cell surface which attaches to the recipiant cell.&nbsp;&nbsp;&nbsp;The pilus pulls the recipient call to the donor so that they can have direct transfer by temporarily joining membranes.<br>The donor cell unwinds it&nbsp;[[DsDNS|double stranded DNA]]&nbsp;plasmid and one of the strands are transfered from the donor to recipiant.<br>Both cells the split apart and synthesises (<u>Semi-conservative replication</u>) the complementory strand so that&nbsp;both&nbsp;donor and recipiant cells&nbsp;now have identicle plasmids.  
+
Bacteria of donor cell has a [[Pilus|Pilus]] (protein hair like appendage) on its cell surface which attaches to the recipiant cell.&nbsp;&nbsp;&nbsp;The pilus pulls the recipient call to the donor so that they can have direct transfer by temporarily joining membranes.<br>The donor cell unwinds it&nbsp;[[DsDNS|double stranded DNA]]&nbsp;plasmid and one of the strands are transfered from the donor to recipient.<br>After which both cells&nbsp;separate and synthesise&nbsp;(<u>Semi-conservative replication</u>) the complementory strand so that&nbsp;both&nbsp;donor and recipiant cells&nbsp;now have identicle plasmids.  
  
The ability to form pili comes from the presence of a fertility or F&nbsp;factor;&nbsp;which is a molecule of DNA that codes for the genes to produce pili. The donor cell which has the F factor is considered to be the F<sup>+</sup> cell and the recipient is considered the F<sup>-</sup> cell. Before the transfer of genes can take place, the F<sup>+</sup> cell must&nbsp;first conjugate with the F<sup>-</sup> cell and transfer a copy of the F factor to it. This will then allow the formerly F<sup>- </sup>cell to conjugate and transfer genes because it has become a F<sup>+</sup> cell.<ref name="null">Snustad P. and Simmons M.J.(2010) Principles of Genetics. 5th Edition. P 192-195 Hoboken: John Wiley &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Sons</ref>  
+
The ability to form pili comes from the presence of a fertility or F&nbsp;factor;&nbsp;which is a molecule of DNA that codes for the genes to produce pili. The donor cell which has the F factor is considered to be the F<sup>+</sup> cell and the recipient is considered the F<sup>-</sup> cell. Before the transfer of genes can take place, the F<sup>+</sup> cell must&nbsp;first conjugate with the F<sup>-</sup> cell and transfer a copy of the F factor to it. This will then allow the formerly F<sup>- </sup>cell to conjugate and transfer genes because it has become a F<sup>+</sup> cell.<ref name="null">Snustad P. and Simmons M.J.(2010) Principles of Genetics. 5th Edition. P 192-195 Hoboken: John Wiley &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Sons</ref>  
  
 
<br>
 
<br>
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Transduction is also known as Phage mediated DNA transfer  
 
Transduction is also known as Phage mediated DNA transfer  
  
[[Bacteriophage|Bacteriophages]] ([[Virus|Virus]]) inject cells with viral DNA which then infects the cell. The cell then produces new phages and breaks open, releasing new [[Phage|phages]] to go and infect other cells.<br>Occationaly when the new phages are being packaged with the viral DNA a mistake is made and it packs host cell [[Chromosmal DNA|chromosmal DNA]]. The phages which do this are called ''''TRANSDUCING PARTICLES''''  
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[[Bacteriophage|Bacteriophages]] ([[Virus|Virus]]) inject cells with viral DNA which then infects the cell causing &nbsp;the cell to produce new phages and break open, releasing the new [[Phage|phages]] which then infect other cells.<br>Occasionally when the new phages are being packaged with the viral DNA, host cell [[Chromosmal DNA|chromosmal DNA]]&nbsp;may be packed in error; such&nbsp;phages are called ''''TRANSDUCING PARTICLES''''. These phages infect other (recipient) cells,&nbsp;injecting host DNA&nbsp;instead of viral DNA into it;&nbsp;the host DNA&nbsp;is then&nbsp;incorporated into the recipients chromosomal DNA, causing it to gain new [[Gene|genes]].
  
These phages then go and infect other (recipiant cells) the host DNA is then injected, opposed to the viral DNA, and the host DNA will be encourperated into the recipiants chromosomal DNA, gaining new [[Gene|genes]].
+
Bacteriophage can be easily detected because they form plaques in a bacteria growth. In the absence of phages, bacterial cells will grow and form a translucent lawn. However when a&nbsp;phage&nbsp;is introduced the bacteria will become infected, replicate more phages, and&nbsp;eventually undergoing cell lysis. This lysis will release the phages into the medium and soon the surrounding cells will also become infected, causing cell lysis&nbsp;to&nbsp;occur in these&nbsp;as well. &nbsp;The bacteria in a localised area will all die (lyse) and&nbsp;create an area where no bacteria exist. These areas are known as plaques.  
 
+
Bacteriophage can be easily detected because they form plaques in a bacteria growth. In the absence of phages, bacterial cells will grow and form a translucent lawn. However when a&nbsp;phage&nbsp;is introduced the bacteria will become infected, replicate more phages, and&nbsp;eventually undergoing cell lysis. This lysis will release the phages into the medium and soon the surrounding cells will also become infected, causing cell lysis&nbsp;to&nbsp;occur is these&nbsp;as well. &nbsp;The bacteria in a localised area will all die (lyse) and&nbsp;create an area where no bacteria exist. These areas are known as plaques.  
+
  
 
=== References  ===
 
=== References  ===
  
 
<references />
 
<references />

Revision as of 20:27, 26 November 2011

There are 3 Types of Gene Transfer in Bacteria [1] namely:

  1. Transformation
  2. Conjugation
  3. Transduction

Contents


Transformation

This is the transfer of genes from Free DNA Molecules in the surrounding medium into a recipiant cell.

The main experiment linked to this was Griffiths work with smooth virilant (Deadly) strain of Streptococcus pneumonia and rough (non-deadly) strain on Mice.
When the smooth strain was treated with heat it was killed and so when injected into the mouse it was not deadly.
However when the 'Heat-Killed' smooth strain and the rough (non-deadly) strain was mixed and injected together the mouse contracted pneumonia. This shows that the smooth strain has 'TRANSFORMED' the rough strain so it becomes deadly when they are mixed. In other words, the rough strain cells had taken up free DNA of the 'killed' smooth strain and thus had produced a protective coating around themselves thus, appearing 'smooth'.

For transformation to occur, the recipient cell has to possess 'competence' which is the ability to take up free DNA fragments by an organism. Competence occurs naturally in many bacteria but it can be induced in bacteria when it does not naturally occur. This can be done by treatment with Calcium Chloride CaCl2 as is the case with Escherichia coli.


Conjugation

Transference of genes via direct cell to cell interactions.
This method involves exchange of  Plasmids (Very uncommon to transfer chromosomal DNA)

Bacteria of donor cell has a Pilus (protein hair like appendage) on its cell surface which attaches to the recipiant cell.   The pilus pulls the recipient call to the donor so that they can have direct transfer by temporarily joining membranes.
The donor cell unwinds it double stranded DNA plasmid and one of the strands are transfered from the donor to recipient.
After which both cells separate and synthesise (Semi-conservative replication) the complementory strand so that both donor and recipiant cells now have identicle plasmids.

The ability to form pili comes from the presence of a fertility or F factor; which is a molecule of DNA that codes for the genes to produce pili. The donor cell which has the F factor is considered to be the F+ cell and the recipient is considered the F- cell. Before the transfer of genes can take place, the F+ cell must first conjugate with the F- cell and transfer a copy of the F factor to it. This will then allow the formerly F- cell to conjugate and transfer genes because it has become a F+ cell.[2]


Transduction

Transduction is also known as Phage mediated DNA transfer

Bacteriophages (Virus) inject cells with viral DNA which then infects the cell causing  the cell to produce new phages and break open, releasing the new phages which then infect other cells.
Occasionally when the new phages are being packaged with the viral DNA, host cell chromosmal DNA may be packed in error; such phages are called 'TRANSDUCING PARTICLES'. These phages infect other (recipient) cells, injecting host DNA instead of viral DNA into it; the host DNA is then incorporated into the recipients chromosomal DNA, causing it to gain new genes.

Bacteriophage can be easily detected because they form plaques in a bacteria growth. In the absence of phages, bacterial cells will grow and form a translucent lawn. However when a phage is introduced the bacteria will become infected, replicate more phages, and eventually undergoing cell lysis. This lysis will release the phages into the medium and soon the surrounding cells will also become infected, causing cell lysis to occur in these as well.  The bacteria in a localised area will all die (lyse) and create an area where no bacteria exist. These areas are known as plaques.

References

  1. Hartl D. L., Ruvolo M. (2012), Genetics: Analysis of genes and genomes, Eight Edition, Jones &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Bartlett learning (Chapter 9 Genetics of Bacteria and their viruses)
  2. Snustad P. and Simmons M.J.(2010) Principles of Genetics. 5th Edition. P 192-195 Hoboken: John Wiley &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Sons
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