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A consensus sequence
it the ideal promotor sequence , its never actually found in [[DNA|DNA]] . It is a sequence of bases downstream of the start site, in ''[[ Escherichia coli|E. coli]] '' it is found at -35 and -10. It was determined experimentally by comparison of known [[Promoter|promoter ]] sequences and selecting the most common bases at each position. Therefore, the closer to the consensus sequence the stronger the [[ Promoter|promoter]] will be. |+|
A consensus sequence ideal sequencein [[DNA|DNA]] in [[|E.coli]]found-35 and -10 . promoter the , the closer to the sequencethe stronger the [[|promoter ]]
Revision as of 16:41, 30 November 2012
A consensus sequence is an ideal promoter sequence in DNA - in E.coli, for example, two are found, a -35 sequence and a -10 sequence. The ideal promoter sequence - the consensus sequence - is never actually found in DNA, and a promoter's strength can be judged by it's similarity to the consensus sequence. The closer a promoter is to the ideal sequence, the stronger it will be and therefore the more mRNA will be produced, which will lead to a greater yield of proteins. The consensus sequence was originally determined by comparison of promoter sequences that were already known and selection of the base that was most common at each position.