DNA polymerase I

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DNA polymerase I is an enzyme that is involved in DNA replication in Escherichia coli. It is part of a group of DNA polymerases that copy DNA; they are highly coordinated in order to replicate whole genomes precisely and rapidly. DNA polymerase I is a slow, non-processive enzyme that fills the gaps between Okazaki fragments of the lagging strand by Watson-Crick base pairing. The enzyme has two additional activites:

  1. 5’→3’ exonuclease activity to remove RNA primers ahead of the polymerase site [1].
  2. 3’→5’ exonuclease activity for proof-reading and accuracy.


  1. Berg J, Tymoczko J, Stryer L (2007) Biochemistry, 6th edition, New York: W.H. Freeman, p. 801
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