Dideoxy sequencing

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(Created page with "Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the s...")
 
 
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Dideoxy sequencing,also known as the chain terminating method or sanger sequencing , is a DNA aequencing method created by Biochemist Fred Sanger and is used in determining the sequence of neuclotides in DNA<ref>This is a reference to the website AK lectures which also has links to the YouTube video i also used.
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Dideoxy sequencing, also known as the chain terminating method or [[Sanger_Method|Sanger]] sequencing, is a [[DNA|DNA]] sequencing method created by Biochemist [[Frederick_Sanger|Fred Sanger]] and is used in determining the sequence of [[nucleotides|nucleotides]] in [[DNA|DNA]]<ref>This is a reference to the website AK lectures which also has links to the YouTube video I also used. http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii</ref>.The basis of this method is down to the incorporation of the [[molecule|molecule]] [[ddNTP|2',3'-dideoxynucleoside triphosphate]]&nbsp;([[ddNTP|ddNTP]]). This molecule lacks a [[hydroxyl group|hydroxyl group]] so [[DNA polymerase|DNA polymerase]] can no longer form a [[phosphodiester bond|phosphodiester bond]]<ref>https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing</ref>. This stops DNA replication.  
http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii</ref>.The basis of this method is down to the incorperation of the meolecule&nbsp; 2',3'-dideoxynucleoside triphosphate(ddNTP).This molecule lacks a hydroxyl group so DNA polymerase can no longer form a phosphodiester bond<ref>https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing</ref>.This stops DNA replication.  
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'''References'''
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=== References ===
  
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Latest revision as of 09:29, 10 December 2018

Dideoxy sequencing, also known as the chain terminating method or Sanger sequencing, is a DNA sequencing method created by Biochemist Fred Sanger and is used in determining the sequence of nucleotides in DNA[1].The basis of this method is down to the incorporation of the molecule 2',3'-dideoxynucleoside triphosphate (ddNTP). This molecule lacks a hydroxyl group so DNA polymerase can no longer form a phosphodiester bond[2]. This stops DNA replication.

References

  1. This is a reference to the website AK lectures which also has links to the YouTube video I also used. http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii
  2. https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing
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