Dideoxy sequencing, also known as the chain terminating method or Sanger sequencing, is a DNA sequencing method created by Biochemist Fred Sanger and is used in determining the sequence of nucleotides in DNA.The basis of this method is down to the incorporation of the molecule 2',3'-dideoxynucleoside triphosphate (ddNTP). This molecule lacks a hydroxyl group so DNA polymerase can no longer form a phosphodiester bond. This stops DNA replication.
- ↑ This is a reference to the website AK lectures which also has links to the YouTube video I also used. http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii
- ↑ https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing