Dideoxy sequencing

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Dideoxy sequencing, also known as the chain terminating method or Sanger sequencing, is a DNA sequencing method created by Biochemist Fred Sanger and is used in determining the sequence of nucleotides in DNA[1].The basis of this method is down to the incorporation of the molecule 2',3'-dideoxynucleoside triphosphate (ddNTP). This molecule lacks a hydroxyl group so DNA polymerase can no longer form a phosphodiester bond[2]. This stops DNA replication.

References

  1. This is a reference to the website AK lectures which also has links to the YouTube video I also used. http://www.aklectures.com/lecture/sanger-sequencing-of-dna-part-ii
  2. https://www.sciencedirect.com/topics/neuroscience/sanger-sequencing
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