Electrophoresis

From The School of Biomedical Sciences Wiki
Revision as of 15:28, 9 October 2014 by 140001613 (Talk | contribs)
Jump to: navigation, search

Electrophoresis is used as a method of separating DNA according to size, using a porous gel as a filter (usually polyacylamide gels are used). There are two separate portions presence across the gel, stacking gel and resolving gel. The stacking gel consists low percentage of polyacrylamide where bigger molecule tends to move slower. While the resolving gel contains higher percentage of polyacrylamide, which the smaller molecule can move faster and further in distance.  By applying an electric current, the molecules of DNA are made to move through the gel towards the positive electrode (anode), with the smaller or more compact strands travelling the fastest. This produces distinct bands of DNA, which can be identified by comparing with a known DNA sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.

The mobility of charges molecules are influenced by:

References

  1. Rob Reed et al (2013) Practical Skills in Biomolecular Sciences 4th edition, Essex, Pearson Education Limited
Personal tools
Namespaces
Variants
Actions
Navigation
Toolbox