Electrophoretic mobility shift assay
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| − | Electrophoretic mobility shift [[ | + | Electrophoretic mobility shift [[Assay|assay]] (also known as the EMSA) is an [[Affinity-electrophoresis|affinity-electrophoresis]] technique used to detect the binding of [[DNA-protein complexes|DNA-protein complexes]]. The Electrophoretic mobility is normally reduced when the [[DNA|DNA]] cognates to [[Protein|protein]], causing a consequent shift in the region of the fragment band. This method is usually used to detect the [[Transcription factor|transcription factor]] in protein, using DNA fragments containing regulatory element <ref>p.286-287 Molecular Cell Biology book.Authors. H.Lodish,A.Berk,C.A.Kaiser,M.Krieger,M.P.Scott,A.Bretscher,H.Ploegh</ref>. |
=== References === | === References === | ||
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Latest revision as of 12:40, 16 November 2017
Electrophoretic mobility shift assay (also known as the EMSA) is an affinity-electrophoresis technique used to detect the binding of DNA-protein complexes. The Electrophoretic mobility is normally reduced when the DNA cognates to protein, causing a consequent shift in the region of the fragment band. This method is usually used to detect the transcription factor in protein, using DNA fragments containing regulatory element [1].
References
- ↑ p.286-287 Molecular Cell Biology book.Authors. H.Lodish,A.Berk,C.A.Kaiser,M.Krieger,M.P.Scott,A.Bretscher,H.Ploegh
