Electrophoretic mobility shift assay

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Electrophoretic mobility shift [[assay|assay]] (also known as the EMSA) is an [[affinity-electrophoresis|affinity-electrophoresis]] technique used to detect the binding of [[DNA-protein complexes|DNA-protein complexes]].The Electrophoretic mobility is normally reduced when the [[DNA|DNA]] cognates to [[protein|protein]], causing a consequent shift in the region of the fragment band. This method is usually used to detect the [[transcription factor|transcription factor]] in protein, using DNA fragments containing regulatory element <ref>p.286-287 Molecular Cell Biology book.Authors. H.Lodish,A.Berk,C.A.Kaiser,M.Krieger,M.P.Scott,A.Bretscher,H.Ploegh</ref>.  
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Electrophoretic mobility shift [[Assay|assay]] (also known as the EMSA) is an [[Affinity-electrophoresis|affinity-electrophoresis]] technique used to detect the binding of [[DNA-protein complexes|DNA-protein complexes]]. The Electrophoretic mobility is normally reduced when the [[DNA|DNA]] cognates to [[Protein|protein]], causing a consequent shift in the region of the fragment band. This method is usually used to detect the [[Transcription factor|transcription factor]] in protein, using DNA fragments containing regulatory element <ref>p.286-287 Molecular Cell Biology book.Authors. H.Lodish,A.Berk,C.A.Kaiser,M.Krieger,M.P.Scott,A.Bretscher,H.Ploegh</ref>.  
  
 
=== References  ===
 
=== References  ===
  
 
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Latest revision as of 12:40, 16 November 2017

Electrophoretic mobility shift assay (also known as the EMSA) is an affinity-electrophoresis technique used to detect the binding of DNA-protein complexes. The Electrophoretic mobility is normally reduced when the DNA cognates to protein, causing a consequent shift in the region of the fragment band. This method is usually used to detect the transcription factor in protein, using DNA fragments containing regulatory element [1].

References

  1. p.286-287 Molecular Cell Biology book.Authors. H.Lodish,A.Berk,C.A.Kaiser,M.Krieger,M.P.Scott,A.Bretscher,H.Ploegh
 
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