Electroporation

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Electroporation is a method used to insert a recombinant plasmid into a bacterial cell using high voltage electrical pulses. 
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[[Image:E_coli-ed01.jpg|right]]Electroporation is a method used to insert a recombinant [[Plasmid|plasmid]] into a [[Bacterial cell|bacterial cell]] using high voltage electrical pulses<ref>http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html</ref>.  
  
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In molecular biology, electroporation is commonly used for cell [[Transfection|transfection]] or [[Transformation|transformation]] of non-viral [[DNA|DNA]] transfer of [[Bacteria|bacteria]], [[Yeast|yeast]], and plant plastids. Electroporation is also highly effective for the introduction of foreign [[Genes|genes]] into tissue culture cells, especially mammalian cells<ref>Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/</ref>.
  
==== <br> '''References'''  ====
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<sup></sup>  
  
1)&nbsp;http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html
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=== <sup></sup>Example of electroporation: ===
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''E. coli'' plasmid insertion with CaCl<sub>2</sub> on ice with heat shock.<br>
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=== References  ===
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<references /><br>

Latest revision as of 18:05, 16 November 2017

E coli-ed01.jpg
Electroporation is a method used to insert a recombinant plasmid into a bacterial cell using high voltage electrical pulses[1].

In molecular biology, electroporation is commonly used for cell transfection or transformation of non-viral DNA transfer of bacteria, yeast, and plant plastids. Electroporation is also highly effective for the introduction of foreign genes into tissue culture cells, especially mammalian cells[2].

Example of electroporation:

E. coli plasmid insertion with CaCl2 on ice with heat shock.

References

  1. http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html
  2. Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/

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