G-banding

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'''G-banding''' is one of the chromosome staining techniques that results in banding patterns on chromosomes. It uses the chemical dye, Giemsa, to create distinct chromosome-specific patterns called G-bands. It allows the identification of each chromosome, as well as its different regions, relative to its banding pattern.<ref name="G-banding">Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.</ref>  
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G-banding is one of the [[chromosome|chromosome]] staining techniques that result in banding patterns on chromosomes. It uses the chemical dye, [[Giemsa|Giemsa]], to create distinct chromosome-specific patterns called G-bands. It allows the identification of each chromosome, as well as its different regions, relative to its banding pattern<ref name="G-banding">Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's Genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.</ref>.
  
The method for G-banding begins with subjecting the chromosome to a treatment involving trypsin (a proteolytic enzyme), and then staining it with Giemsa. This produces dark-stained bands at the regions with lower G-C content. <ref name="Method ">Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.</ref>
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The method for G-banding begins with subjecting the chromosome to a treatment involving [[trypsin|trypsin]] (a [[proteolytic enzyme|proteolytic enzyme]]), and then staining it with Giemsa. This produces dark-stained bands at the regions with lower [[G-C content|G-C content]]<ref name="Method">Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's Genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.</ref>.
 
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=== References  ===
 
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Latest revision as of 13:13, 27 November 2018

G-banding is one of the chromosome staining techniques that result in banding patterns on chromosomes. It uses the chemical dye, Giemsa, to create distinct chromosome-specific patterns called G-bands. It allows the identification of each chromosome, as well as its different regions, relative to its banding pattern[1].

The method for G-banding begins with subjecting the chromosome to a treatment involving trypsin (a proteolytic enzyme), and then staining it with Giemsa. This produces dark-stained bands at the regions with lower G-C content[2].

References

  1. Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's Genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.
  2. Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's Genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.
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