G-banding

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G-banding is one of the chromosome staining techniques that result in banding patterns on chromosomes. It uses the chemical dye, Giemsa, to create distinct chromosome-specific patterns called G-bands. It allows the identification of each chromosome, as well as its different regions, relative to its banding pattern[1].

The method for G-banding begins with subjecting the chromosome to a treatment involving trypsin (a proteolytic enzyme), and then staining it with Giemsa. This produces dark-stained bands at the regions with lower G-C content[2].

References

  1. Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's Genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.
  2. Lewin, B., Krebs, J., Kilpatrick, S. and Goldstein, E. (2014). Lewin's Genes XI. Burlington: Jones and Bartlett Learning, pp.204, 205.
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