# Genetic mutation

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-rearrangement of bases. | -rearrangement of bases. | ||

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To investigate the events leading to mutation we can look at mutagenesis process. There are three types: | To investigate the events leading to mutation we can look at mutagenesis process. There are three types: | ||

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-directed (in vitro change to genome). | -directed (in vitro change to genome). | ||

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We can calculate the rate of mutation occurance using an equation from Poisson distribution: | We can calculate the rate of mutation occurance using an equation from Poisson distribution: | ||

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where P<sub>0</sub> is the probability of seeing no mutations, N is the number of cells per culture and m is the mutation rate. | where P<sub>0</sub> is the probability of seeing no mutations, N is the number of cells per culture and m is the mutation rate. | ||

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+ | '''References:''' | ||

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+ | Genetics, 1987, Geoffrey Zubay | ||

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+ | Encyclopedia of Genetics,Volume 3, 2002, S.Brenner,J.H. Miller |

## Revision as of 12:10, 23 November 2012

**Genetic mutation- **this term indicates an alteration to base sequence in DNA of an organism which results in a mutant allele of a gene i.e. an allele that occurs in less than 1% of population.The change is heritable and can be passed on to offspring if fixed into the parental genome. Mutations can vary depending on the type of genomic alteration:

-addition

-deletion

-substitution

-rearrangement of bases.

To investigate the events leading to mutation we can look at mutagenesis process. There are three types:

-spontaneous (no mutation causing agent)

-induced (a foreign genome introduced e.g.in the laboratory)

-directed (in vitro change to genome).

We can calculate the rate of mutation occurance using an equation from Poisson distribution:

**P _{0}=e^{-mN}**

^{}

where P_{0} is the probability of seeing no mutations, N is the number of cells per culture and m is the mutation rate.

**References:**

Genetics, 1987, Geoffrey Zubay

Encyclopedia of Genetics,Volume 3, 2002, S.Brenner,J.H. Miller