Immunolabelling

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Immunolebelling is a technique used to detect [[Proteins|proteins]] this could in a cell or in an expertiment such as [[Western Blotting|Western Blotting]]. An [[Antibody|antibody]] that will recongise that your desired protein is added, in case of a [[Cell|cell]] a method to get the antibody into the cell must be deveopled. The antibody ([[Primary antibody|primary antibody]])&nbsp;will specifically bind to the [[Antigen|antigen]]. In a Western Blottting experiment this take about an one hour and half on a moving plate. Then you add a [[Secondary antibody|secondary antibody]] which recongises the primary antibody as a antigen. The secondary antibody is conjugated with [[Fluorescent marker|fluorescent marker]]. So the location of your protein can be detected using this technique <ref>Giepmans et al (2006) et al. 312 , 217-223</ref>.  
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Immunolebelling is a technique used to detect [[Proteins|proteins]] either in a cell or in an experiment&nbsp;such as [[Western Blotting|Western Blotting]]. An [[Antibody|antibody]]&nbsp;will recongise when the desired protein is added, in the case of a [[Cell|cell]] a method must be devised to get the antibody inside. The antibody ([[Primary antibody|primary antibody]]) specifically binds to the [[Antigen|antigen]]. In a Western Blottting experiment this takes about an hour and half on a moving plate. Then you add a [[Secondary antibody|secondary antibody]] which recongises the primary antibody as an antigen. The secondary antibody is conjugated with a&nbsp;[[Fluorescent marker|fluorescent marker]], so the location of your protein can be detected using this technique.  
  
 
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=== References  ===
  
 
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Revision as of 21:28, 22 October 2018

Immunolebelling is a technique used to detect proteins either in a cell or in an experiment such as Western Blotting. An antibody will recongise when the desired protein is added, in the case of a cell a method must be devised to get the antibody inside. The antibody (primary antibody) specifically binds to the antigen. In a Western Blottting experiment this takes about an hour and half on a moving plate. Then you add a secondary antibody which recongises the primary antibody as an antigen. The secondary antibody is conjugated with a fluorescent marker, so the location of your protein can be detected using this technique.

References

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