Insulin

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Insulin is a hormone. Its main function is the regulation of blood sugar levels, by causing the liver and muscles to increase uptake of glucose [1]. Insulin is produced from a single gene which codes for the peptide proinsulin; a precursor moleculeMutations in this gene can result in a faulty protein; causing type 1 diabetes or a possible predisposition to type 2 diabetes [2][3].

Insulin regulates the blood glucose levels in different ways. It enhances the glucose transport at a cellular level by stimulation of the glucose transporter (GLUT) family. 

Insulin also has an effect on gene expression which is up or down regulated in the homeostasis process to maintain the optimum blood glucose levels.

Insulin is released by the beta-cells of the pancreas.

Contents

History

Frederick sanger's pioneering research led him to discover the amino acid sequence of the insulin  in 1953.[4]

Insulin stimulates glycogen synthesis

When blood sugar levels are high, insulin binds to a tyrosine kinase receptor. Binding of insulin triggers a phosphorylation cascade, preventing phosphorylation of glycogen synthase as this inactivates it's activity [5].

Insulin acts antagonistically to the hormone glucagon, which acts on glycogen storage in response to low blood sugar levels [6]. This serves as an effective homeostasis mechanism. 

Origin of structure

Insulin is extracted and purified by crystallisation from the beta cells of Langerhands of pancreas in prok and beef. It is made by using E.coli in recombinant DNA technology (biosynthetically) or enzymatic modification of porcine material (semisynthetically) [7].

Forming the structure of Insulin 

Insulin has two peptide chains, named a and b which are linked together by disulphide bonds between each of the two chains. Proinsulin is the molecule that insulin is synthesised from, unlike insulin it contains three peptide chains (a, b and c). Proinsulin folds to the correct shape through the formation of the disulphide bonds between the a and b peptide chains and then the peptide chain, c, located between the a and the b chains is removed in order to complete the structure of insulin. 

Phsical properties

The physical property of insulin substance is white in color and it is in the form of white crystalline powder because it is purified by crystallisation, hence 'crystalline'. It is soluble in water and dilute solution of mineral acids and insoluble in alcohol.chloroform and ether [8].

References

  1. http://www.ncbi.nlm.nih.gov/omim/176730
  2. http://www.ncbi.nlm.nih.gov/omim/176730
  3. http://www.ncbi.nlm.nih.gov/pubmed/20806184
  4. Lubert Stryer (1995). Biochemistry. 4th ed. New York : W.H. Freeman . 25.
  5. Berg J., Tymoczko J and Stryer L. (2007) Biochemistry, 6th edition, New York: WH Freeman.fckLR
  6. Berg J., Tymoczko J and Stryer L. (2007) Biochemistry, 6th edition, New York: WH Freeman.fckLR
  7. http://www.inchem.org/documents/pims/pharm/insulin.htm#SectionTitle:3.2 Chemical structure
  8. http://www.inchem.org/documents/pims/pharm/insulin.htm#SectionTitle:3.2 Chemical structure
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