Ligase

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Ligase is a type of enzyme that forms a link between carbon and either: carbon, sulphur, oxygen, or nitrogen; using the hydrolysis of ATP and its high energy bond to drive formation of the new covelant bond [1].There are various types of ligases; one of the most important ones is DNA ligase which joins DNA fragments via phosphodiester bonds[2] and is used in processes such as DNA replication where Okazaki fragments need to be annealed in order to complete the formation of the laggin strand. 

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