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Carbon bonds can be cleaved in oxidation and hydrolysis reactions. They can also be cleaved when two substrates form one product or when one substrate forms two products. When the CO or H2O is released a double bond will form. Lyases are the enzymes which catalyse these reactions.

An example which illistrates this is the conversion of fructose 1,6-bisphosphate (6 carbon compound) into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate (both 3 carbon compounds).

Jeremy M.Berg, John L. Tymoczko, Gregory J. Gatto, Lupert Stryer (2011). Biochemistry. 8th ed. W. H. Freeman; Eigth edition (1600). p442.

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