MRNA

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Pre mRNA is the exact copy of the DNA sequence, containing [[Intron|introns]] and [[Exon|exons]]. This is not helpful in cloning experiments as bacteria and other [[Prokaryotes|prokaryotes]] do not have the capibility to process this form of mRNA and remove the introns.  
 
Pre mRNA is the exact copy of the DNA sequence, containing [[Intron|introns]] and [[Exon|exons]]. This is not helpful in cloning experiments as bacteria and other [[Prokaryotes|prokaryotes]] do not have the capibility to process this form of mRNA and remove the introns.  
  
Mature mRNA is the product when pre mRNA undergoes ''processing''. This involves removing the non-coding introns, joining the coding exons together, and adding a [[Poly A tail|poly A tail to]] the[[3' end| 3' end]], to form a new strand with an uninterrupted sequence which is then translated into amino acids and proteins. Mature mRNA is required for protein cloning in the lab as it does not require any further processing by the prokaryotes.  
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Mature mRNA is the product when pre mRNA undergoes ''processing''. This involves a process called 'splicing' which removes the non-coding introns. Processing also encompasses joining the coding exons together, and adding a [[Poly A tail|poly A tail to]] the[[3' end| 3' end]], to form a new strand with an uninterrupted sequence, which is then translated into amino acids and proteins. Mature mRNA is required for protein cloning in the lab as it does not require any further processing by the prokaryotes.  
  
mRNA can also be used to make [[CDNA|cDNA]] (copy DNA). [[Reverse transcriptase|Reverse transcriptase]] is used to convert mRNA into [[CDNA|cDNA]].
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mRNA can also be used to make [[CDNA|cDNA]] (copy DNA). An enzyme called [[Reverse transcriptase|Reverse transcriptase]] is used to convert mRNA into [[CDNA|cDNA]].

Revision as of 21:44, 20 October 2014

Messenger RNA (mRNA) is an important form of RNA, both in the body and in the lab. Physiologically, it is used as the coding template for proteins, i.e. it directly transcribes the sequence of nucleotides from the DNA template (in a process known as transcription), forming a second complementary strand which is later processed by rRNA and tRNA to form a new DNA strand.

There are two forms of mRNA that can be found in eukaryotic cells: pre-mRNA and mature mRNA.

Pre mRNA is the exact copy of the DNA sequence, containing introns and exons. This is not helpful in cloning experiments as bacteria and other prokaryotes do not have the capibility to process this form of mRNA and remove the introns.

Mature mRNA is the product when pre mRNA undergoes processing. This involves a process called 'splicing' which removes the non-coding introns. Processing also encompasses joining the coding exons together, and adding a poly A tail to the 3' end, to form a new strand with an uninterrupted sequence, which is then translated into amino acids and proteins. Mature mRNA is required for protein cloning in the lab as it does not require any further processing by the prokaryotes.

mRNA can also be used to make cDNA (copy DNA). An enzyme called Reverse transcriptase is used to convert mRNA into cDNA.

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