Mass Spectrometry in the process in where proteins can be identified. Whether it be a single protein, or a collections of proteins, obatined after procedures such as gel electrophoresis or by column chromatography.The proteins in the sample are firstly broken down into smaller peptides, where they are ionized creating a postive charge. These ionized peptides are accelerated via an electric field towards a decetor. The mass to charge ratio of these ions affect the time it takes for them to reach the dector, large peptides moving slower and higher charged molecules move faster. The masses of the peptides obtained by the detector can then be measured and compared to other well known peptides in a database, determing the genomic seqeunce and ultimately the protein.
- ↑ Alberts et al (2008) Molecular Biology Of The Cell, 5th Edition