Maximum Reaction Velocity

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Maximum Velocity (Vmax) occurs when all enzymes are working at they're maximum speed at high substrate concentration. The enzymes are said to saturated by the substrate; increasing the substrate concentration will not increase the velocity of the reaction[1]. Maximum velocity can be determined from a plot of rate of product formation (initial velocity) against substrate concentration. 

References

  1. ↑ Berg J, Tymoczko J, Gatto G, Stryer L. Biochemistry. 8th ed, New York: Freeman. 2015. p288-230
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