Michaelis constant

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Km = Michaelis constant

Km = the value of [S] that causes V= ½ Vmax *

The units of Km are M, concentration

Km indicates the affinity of the enzyme for its substrate and thus the stability of the Enzyme-Substrate Complex.

High Km demonstrates a low affinity; Low Km demonstrates a high affinity.

Velocity is related to Kthrough the Michaelis & Menten equation: v = (Vmax [S])/(Km + [S])

[S] = substrate concentration

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