Km = Michaelis constant
Km = the value of [S] that causes V= ½ Vmax *
The units of Km are M, concentration
Km indicates the affinity of the enzyme for its substrate and thus the stability of the Enzyme-Substrate Complex.
High Km demonstrates a low affinity; Low Km demonstrates a high affinity.
Velocity is related to Km through the Michaelis & Menten equation: v = (Vmax [S])/(Km + [S])
- Vmax = maximum rate of reaction achievable for the enzyme under given conditions, only occurs at infinite substrate concentration
[S] = substrate concentration