# Michaelis menten equation

(Difference between revisions)
 Revision as of 09:00, 10 January 2011 (view source)Nnjm2 (Talk | contribs)← Older edit Latest revision as of 10:36, 22 October 2014 (view source) Line 7: Line 7: '''V''''''max''' is the maximum rate of an [[Enzyme|enzyme]] reaction, occurs when all substrate is saturated.
'''V''''''max''' is the maximum rate of an [[Enzyme|enzyme]] reaction, occurs when all substrate is saturated.
− '''Km''' is the Michaelis-Menten constant and is the substrate concentration at half Vmax .
+ '''Km''' is the Michaelis-Menten constant and is the substrate concentration at half Vmax . (Higher the Km value, lower the affinity)
Vmax and Km can also be shown on a graph, the graph which shows this best is a '''double reciprocal plot '''([[Lineweaver-Burk|Lineweaver-Burk]] plot). You can obtain the results by plotting 1/V against 1/[S] Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163. Vmax and Km can also be shown on a graph, the graph which shows this best is a '''double reciprocal plot '''([[Lineweaver-Burk|Lineweaver-Burk]] plot). You can obtain the results by plotting 1/V against 1/[S] Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163.

## Latest revision as of 10:36, 22 October 2014

The Michaelis-Menten equation is used to work out the rate of enzyme reactions and is written as follows:

V = Vmax [S]

Km + [S]

Vmax is the maximum rate of an enzyme reaction, occurs when all substrate is saturated.

Km is the Michaelis-Menten constant and is the substrate concentration at half Vmax . (Higher the Km value, lower the affinity)

Vmax and Km can also be shown on a graph, the graph which shows this best is a double reciprocal plot (Lineweaver-Burk plot). You can obtain the results by plotting 1/V against 1/[S] .