Michaelis menten equation

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The Michaelis-Menten equation is used to work out the rate of enzyme reactions and is written as follows:

                                                     V = Vmax [S]

                                                           Km + [S]

Vmax is the maximum rate of an enzyme reaction, occurs when all substrate is saturated.

Km is the Michaelis-Menten constant and is the substrate concentration at half Vmax . (Higher the Km value, lower the affinity)

Vmax and Km can also be shown on a graph, the graph which shows this best is a double reciprocal plot (Lineweaver-Burk plot). You can obtain the results by plotting 1/V against 1/[S] [1].


  1. Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163
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