Michaelis menten equation
The Michaelis-Menten equation is used to work out the rate of enzyme reactions and is written as follows:
V = Vmax [S]
Km + [S]
Vmax is the maximum rate of an enzyme reaction, occurs when all substrate is saturated.
Km is the Michaelis-Menten constant and is the substrate concentration at half Vmax.
Vmax and Km can also be shown on a graph, the graph which shows this best is a double reciprocal plot (Lineweaver- Burk plot). You can obtain the results by plotting 1/V against 1/[S] .
- ↑ Molecular Biology of the Cell, Alberts et al., 5th Edition (2007) Garland Science, New York Chapter 3 p162-163