Modifying enzymes

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Modifying [[Enzyme|enzymes]] remove or add specific chemical groups. An example is, [[Alkaline_phosphatase|alkaline phosphatase]]&nbsp;(from [[E. coli|''E. coli'']], calf intestinal tissue, or arctic shrimp), which removes the phosphate group present at the 5' terminus of a [[DNA|DNA]] [[Molecule|molecule]]<ref>T.A.Brown (2010). Gene cloning &amp;amp;amp;amp;amp; DNA analysis: an introduction. 6th ed. Hoboken : Wiley-Blackwell. p49-50</ref>.<br>
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Modifying [[Enzyme|enzymes]] remove or add specific chemical groups. An example is, [[Alkaline phosphatase|alkaline phosphatase]] (from [[E. coli|''E. coli'']], calf intestinal tissue, or arctic shrimp), which removes the phosphate group present at the 5' terminus of a [[DNA|DNA]] [[Molecule|molecule]]<ref>T.A.Brown (2010). Gene cloning and DNA analysis: an introduction. 6th ed. Hoboken: Wiley-Blackwell. p49-50</ref>.  
  
 
Modifying enzymes are useful to protect the DNA from being cleaved by a [[Restriction enzyme|restriction enzyme]]<ref>http://www.reference.md/files/D015/mD015280.html</ref>.  
 
Modifying enzymes are useful to protect the DNA from being cleaved by a [[Restriction enzyme|restriction enzyme]]<ref>http://www.reference.md/files/D015/mD015280.html</ref>.  
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=== References  ===
 
=== References  ===
  
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Latest revision as of 10:07, 8 December 2018

Modifying enzymes remove or add specific chemical groups. An example is, alkaline phosphatase (from E. coli, calf intestinal tissue, or arctic shrimp), which removes the phosphate group present at the 5' terminus of a DNA molecule[1].

Modifying enzymes are useful to protect the DNA from being cleaved by a restriction enzyme[2].

References

  1. T.A.Brown (2010). Gene cloning and DNA analysis: an introduction. 6th ed. Hoboken: Wiley-Blackwell. p49-50
  2. http://www.reference.md/files/D015/mD015280.html
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