Modifying enzymes

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Modifying enzymes remove or add specific chemical groups. An example is, alkaline phosphatase (from E. coli, calf intestinal tissue, or arctic shrimp), which removes the phosphate group present at the 5' terminus of a DNA molecule[1].

Modifying enzymes are useful to protect the DNA from being cleaved by a restriction enzyme[2].

References

  1. T.A.Brown (2010). Gene cloning and DNA analysis: an introduction. 6th ed. Hoboken: Wiley-Blackwell. p49-50
  2. http://www.reference.md/files/D015/mD015280.html
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