Morpholino

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== '''Description''' ==
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=== Description ===
  
*Small oligomer of synthetic nucleotide analogues that block gene expression  
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*Small oligomer of synthetic nucleotide analogues that block gene expression.
*25bp length
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*25 bp length
  
== '''Function''' ==
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=== Function ===
  
 
*attaches to mRNA, pre/mature, to cause gene knockdown  
 
*attaches to mRNA, pre/mature, to cause gene knockdown  
 
*Used as gene knockdown tool on zebra fish<ref>Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.</ref>
 
*Used as gene knockdown tool on zebra fish<ref>Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.</ref>
  
On mature mRNA:  
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==== On mature mRNA: ====
  
 
*attaches to AUG blocking ribosome interaction
 
*attaches to AUG blocking ribosome interaction
  
On pre-mRNA:  
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==== On pre-mRNA: ====
  
*sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br>
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*sits on splice site, an intron-exon junction, making a double-stranded RNA, blocking spliceosome which will create truncated/no protein<br>
  
<br>
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=== References  ===
  
== '''References'''<br>  ==
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<references />
 
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<references /><br>
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<br>
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Revision as of 17:15, 16 November 2017

Contents

Description

Function

On mature mRNA:

On pre-mRNA:

References

  1. Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.
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